Tianlong P115H Coxsackie A6/A10 PCR Detection Kit PCR Kit

• Customization: Available
• Type: IVD Reagent
• Product Type: PCR Kit
• Model NO.: P115H
• Certification: CE
• Specimen: Oropharyngeal swabs
• Target Pathogene: Coxsackie A6/A10
• Storage & Validity: -25ºC~-15ºC for 12 months
• Transport Package: Paper Carton with Icy Bag
• Specification: 32T/Kit
• Trademark: Tianlong
• Origin: China
• HS Code: 3822009000
• Production Capacity: 5000000 Test/Year

Product Description

Product Name: 
Coxsackie A6/A10 Nucleic Acid Detection Kit (Fluorescence PCR Method)
 

Spec: 
32T/Kit

Description:

Hand, foot, and mouth disease (HFMD) is an infectious disease caused by enteroviruses, which characterizes by fever and vesicular eruptions on the hands, feet, and mouth. There are more than 20 kinds of enteroviruses that cause HFMD. Among them, enterovirus 71 and coxsackie A16 are the most common pathogen. But in recent years, non-EV71 and non-CA16 EVs have become important pathogens causing HFMD, among which Coxsackievirus group A Type 6 (CA6) and Coxsackievirus Group A Type 10 (CA10) are important pathogens.

Tianlong Coxsackie A6/A10 Nucleic Acid Detection Kit is intended for the qualitative detection of Coxsackie A6/A10 Virus nucleic acid by real-time reverse transcription polymerase chain reaction (Real-time RT-PCR) method, assisting in the diagnosis and treatment of Coxsackie A6/A10 virus infection patients and public healthcare management.

Features:

Innovative 3-in-1 tube PCR (Upgraded single-tube PCR): Innovative single-tube PCR, with reaction solution, primers and PCR enzymes in one tube, minimize time of sample adding. PCR detection within 40 minutes
High Precision: The precision values of intra and inter Ct values were all <5%
Specificity: No cross reaction with other common respiratory disease related pathogens.

Specifications

Product Name	Coxsackie A6/A10 Nucleic Acid Detection Kit (Fluorescence PCR Method)
Cat.No	P115H
Specification	32T/Kit
Specimen	Oropharyngeal swabs
Sensitivity	500 copies/mL
Target Pathogene	Coxsackie A6/A10
Storage & Validity	-25ºC~-15ºC for 12 months
Applicable Equipment	Instruments with FAM, VIC (HEX), CY5 channels such as Applied Biosystems™ 7500 Real-Time PCR Systems and Tianlong Gentier Real-time PCR Systems
Certificate	CE  marked

 

Intended Use:

The kit is used for in vitro qualitative detection of CA6 nucleic acid and CA10 nucleic acid in human throat swab samples.

Coxsackie is an enterovirus divided into two categories: A and B, among which there are 23 types of type A (A) and 6 types of type B (B). All of them are highly infectious. Coxsackie is a common type of virus that infects human body through respiratory and digestive tract. Infected people will appear fever, sneezing, coughing and other cold symptoms.

The laboratory tests of coxsackie include:
1) Coxsackie isolation and detection;
2) Coxsackie specific marker detection;
3) Serological diagnostic test, etc.

This kit uses real-time fluorescent quantitative PCR method.
 

Principle of Test:

The highly conserved sequences of CA6 and CA10 are selected to design specific primers and Taqman probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5'end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3'end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of CA6/CA10 nucleic acid in closed reaction system.
A pair of housekeeping gene-specific primers are selected as the internal standard in the kit and combined with specific probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. The internal control adopts TEXAS RED channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negatives.

Storage Condition & Validity:

All reagents should be stored at -20ºC±5 ºC. The validity period of the reagents is 12 months.

 

Sample Requirements :

1. Sample: human throat swab samples.

2. Collection container: a disposable throat swab sampler approved by the state should be selected.

3. Collection: for specific sampling method, please refer to the "Microbial Specimen Collection Manual".

4. Storage: samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for long-time, but repeated freeze-thaw should be avoided.

5. Transportation: use a foam box with ice to seal for transportation.

 

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