Product Name:
Enterovirus Universal RNA Detection Kit (Fluorescence PCR Method)
Spec:
32T/Kit
Intended Use:
The kit is used for in vitro quantitative detection of enterovirus universal (EU) nucleic acid in human throat swab samples.
Hand, foot and mouth disease (HFMD) is an infectious disease caused by enterovirus, which belongs to the IV RNA virus. There are more than 20 kinds of enteroviruses that cause HFMD. Coxsackie virus group A (type 16, 4, 5, 9, 10), group B (type 2, 5), some echovirus and enterovirus 71 (type 71) are the common pathogens of HFMD. Among them, enterovirus 71 and coxsackie A16 are the most common. The laboratory tests of enterovirus include: 1) Enterovirus isolation and testing; 2) Enterovirus specific marker detection; 3) Serological diagnostic test, etc. This kit uses real-time fluorescent quantitative PCR method. Through clinical verification, this kit can detect enterovirus 71, coxsackie A16, coxsackie A4, coxsackie A5, coxsackie A6, coxsackie A7, coxsackie A9, coxsackie A10, coxsackie B2 and coxsackie B5.
71, coxsackie A16, coxsackie A4, coxsackie A5, coxsackie A6, coxsackie A7, coxsackie A9, coxsackie A10, coxsackie B2 and coxsackie B5.
Principle of Test:
The highly conserved sequences of enterovirus are selected to design specific primers and Taqman probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5'end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3'end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of EU nucleic acid in closed reaction system.
A pair of housekeeping gene-specific primers are selected as the internal standard in the kit and combined with specific probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. The internal control adopts VIC channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negatives.
Storage Condition & Validity:
All reagents should be stored at -20ºC±5 ºC. The validity period of the reagents is 12 months.
Applicable Instrument:
Gentier 96E real time PCR system and Gentier 96R real time PCR system, TL988-IV real time PCR system, ABI7500 real time PCR systems
Sample Requirements :
1. Sample: human throat swab samples.
2. Collection container: a disposable throat swab sampler approved by the state should be selected.
3. Collection: for specific sampling method, please refer to the "Microbial Specimen Collection Manual".
4. Storage: samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for long-time, but repeated freeze-thaw should be avoided.
5. Transportation: use a foam box with ice to seal for transportation.
Tianlong Real Time PCR Test Kit Catalogue:
Tianlong Product Solution:
Audited Supplier 
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