Neisseria Gonorrhoeae (NG) Nucleic Acid Detection Kit (Fluorescence PCR Method)
Product Name:
Neisseria Gonorrhoeae (NG) Nucleic Acid Detection Kit (Fluorescence PCR Method)
Spec:
32T/kit, 96T/kit
Intended Use:
The kit is used for qualitative detection of neisseria gonorrhoeae nucleic acid in male urethral swab samples or female cervical swab samples. Neisseria gonorrhoeae can cause purulent infections of the urogenital tract, such as urethritis and cervicitis.
The laboratory tests of neisseria gonorrhoeae include:
1) Smear examination, preliminary judgment;
2) Neisseria gonorrhoeae culture method;
3) Antigen detection method;
4) Nucleic acid amplification test. The kit developed by our company is suitable for the auxiliary diagnosis of neisseria gonorrhoeae. The test results of this kit are for clinical
reference only and cannot be used as the basis for diagnosis or exclusion of cases.
Principle of Test:
The highly conserved sequences of neisseria gonorrhoeae are selected to design specific primers and Taqman probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5'end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3'end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of neisseria gonorrhoeae nucleic acid in closed reaction system. The internal standard detection system is used in this kit, which can effectively monitor the occurrence of false negatives. A synthetic non-competitive sequence is designed as an internal control template of this kit, which has no interference with the target gene of neisseria gonorrhoeae, then BLAST ratio analysis is performed for this sequence on NCBI website, confirmed that it cannot be found in the NCBI nucleic acid library, no interfere with each other. The primers and probes are designed according to the internal control template, the internal control adopts HEX channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negatives.
Storage Condition & Validity:
All reagents should be stored at -20ºC±5ºC. The validity period of the reagents is 12 months.
The unused reagents can be sequentially cryopreservation without affecting their stability, but it should not exceed three times for repeated thawing and freezing. The opened reagents should be placed at room temperature under the condition of no more than 8 hours. The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days. Production date and expiration date are shown on the packaging label.
Applicable Instrument:
ABI7500 real time PCR systems, TL988-IV real time PCR system, Gentier 96E real time PCR system, Gentier 96R real time PCR system and Gentier 48E real time PCR system.
Sample Requirements:
1. Sample: male urethral swab samples or female cervical swab samples.
2. Collection container: disposable male swabs or female swabs approved by the state should be used. The swabs should include sleeves, cotton swab rods, and stoppers. The cotton swab rods and stoppers should be firmly connected.
3. Collection: for specific sampling method, please refer to the 'Microbial Specimen Collection Manual'.
3.1 Male: clean the urethral orifice first, and the patient is required not to urinate for 2 hours before sampling. Take urethral secretions or a small cotton swab to extend into the urethra 2~4cm, slightly twist the swab to remove the secretion (it should be slightly mucous), place the secretion or cotton swab into a sterile glass tube and seal it for inspection.
3.2 Female: first use a cotton/linen swab to wipe off the excessive secretions of the cervix, use a vaginal dilator to expand the vagina, then exchange for a cotton swab to extend into the cervix and pass through the epithelial junction until the swab head cannot be seen, rotate for 10-20 seconds, take out the swab, put the swab in a sterile glass tube and seal it for inspection.
4. Storage: samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for long-time, but repeated freeze-thaw should be avoided.
5. Transportation: use foam box and ice bag to seal for transportation.
Product Innerside :
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