Product Name:
Influenza A/Influenza B/Human Adenovirus/2019nCoV Nucleic Acid Detection Kit
Spec:
50T/kit
Intended Use:
This kit is intended to be used for the qualitative detection of influenza A virus (Flu-A), influenza B virus (Flu-B), human adenovirus (HAdV) and novel coronavirus (2019ncov), wherein tube A is used to detect influenza A virus, influenza B virus and human adenovirus, while tube B is used to detect the ORF1ab gene and the N protein gene of the novel coronavirus (2019nCoV) and then further determine the infection virus type according to the detection results. However, the detection results are for clinical reference only and cannot be used as the basis for diagnosis or exclusion of any cases alone.
Principle of Test:
This detection kit has designed a set of specific primer and specific probe respectively for the specific genes of influenza A virus (Flu-A), influenza B virus (Flu-B) and human adenovirus (HAdV), and the conservative regions of the ORF1ab gene and the N protein gene of the novel coronavirus (2019nCoV). Such probe can be specifically bound to a section of DNA template in the middle of the primer amplification region. During the PCR extension reaction, the Taq Enzyme's exonuclease activity can cut the 5'-end fluorescent group off the probe, enabling it to dissociate in the reaction system and get rid of the shielding of the 3' -end fluorescent quencher. Then, the 5'-end fluorescent group can receive light stimulation and emit fluorescence that can be detected by the instrument, so that automatic detection for influenza A virus, influenza B virus, human adenovirus and novel coronavirus (2019ncov) can be realized in a completely closed reaction system.
This detection kit selects a pair of housekeeping gene-specific primers as its internal reference for bonding with a specific probe. Such probe can be specifically bound to a section of nucleic acid template in the middle of the primer amplification region. The internal reference uses the ROX/TEXAS RED channel to realize the monitoring of the detection process in a completely closed reaction system, and to monitor the occurrence of false negative effectively.
Storage Condition & Validity:
It shall be stored at -20ºC±5ºC and used within 12 months.
Reagents may continue to be cryopreserved if not used up, which will not affect their stability, but the repeated freezing and thawing of the reagents shall not exceed three times. The reagents shall be kept at room temperature for no more than 8 hours after they are opened. The product shall be transported at -25ºC~8ºC. Simulated transportation experiments show that the transportation conditions will not affect the stability or validity of the product, but the transportation time shall not exceed 7 days. For production date and expiry date of the kit, please refer to the package label.
Applicable Instrument:
Applicable amplification detection instruments include the Applied Biosystems 7500 Real-Time PCR System (real-time fluorescence quantitative PCR detection system), and the Gentier96E and Gentier96R full-automatic medical PCR analysis system (real-time fluorescence quantitative PCR detection system) and Gentier48E full-automatic PCR analysis system (real-time fluorescence quantitative PCR detection system) from Xi'an Tianlong Science and Technology Co., Ltd. All applicable instruments have FAM, VIC (HEX) and ROX/TEXAS RED fluorescent light channels.
Sample Requirements:
1. Specimen: Nasopharyngeal swab, sputum and alveolar lavage fluid.
2. Specimen collection: For specific methods of specimen collection, please refer to the Microbiological Specimen Collection Manual.
3. Storage: Keep at 2-8ºC for no more than 24 hours; keep at -20ºC±5ºC for no more than 3 months; keep at below -70ºC for long-term storage, but repeated freezing and thawing shall be avoided.
4. Transportation: Transport it with a foam box filled with ice bags, and keep the box tightly closed.
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