Product Name:
Human K-ras Gene Mutation Detection Kit (PCR-Melting Curve Method)
Intended Use:
The kit uses genomic DNA extracted from tumor paraffin-embedded tissue sections of human non-small cell lung cancer and colorectal cancer as test samples for qualitative detection of 12 somatic mutations at codon 12 and 13 of K-ras gene .
K-ras gene, located on chromosome 12p12.1, is one of the important oncogenes. It encodes a 21kD KRAS protein and participates in intracellular signal transduction, mainly including RAS/PI3K/PTEN/ Akt and RAS/RAF/MEK/ERK signal transduction pathway. These transduction pathways are currently the hotspots in the research of tumor-targeted drugs. Targeted drugs have pharmacological effects by inhibiting these pathways. Studies have shown that K-ras gene mutations exist in non-small cell lung cancer and colorectal cancer, and mutation of K-ras occurring at codon 12 and 13 which could cause KRAS protein to mutate and remain activated can render the drug ineffective.
According to the 2010 edition of the Chinese Oncology Clinical Practice Guidelines, 101 patients with bronchioloalveolar carcinoma of the lung adenocarcinoma subtype received first-line treatment with erlotinib alone in a retrospective study. The results showed that no one (0/18) achieved remission in K-ras mutation group, while 20 (20/62, 32%) had remission in non-K-ras mutation group (P<0.01). Therefore, the guidelines recommend that patients with non-small cell lung cancer and colorectal cancer should detect mutation of K-ras before using targeted drugs. This product is suitable for patients with non-small cell lung cancer and colorectal cancer before entering the individualized targeted therapy course, and provides auxiliary diagnostic evidence for the individualized medication of clinical tumor-targeted drugs.
| Product Name |
Human K-ras Gene Mutation Detection Kit (PCR-Melting Curve Method) |
| Cat.No |
P112H |
| Specification |
20T/Kit |
| Specimen |
paraffin-embedded tissue sections of human non-small cell lung cancer and colorectal cancer |
| Storage & Validity |
It shall be stored at -20ºC±5ºC in a dark place, and is valid for 12 months. Repeated freezing and thawing shall be avoided. |
| Applicable Equipment |
ABI7500 real time PCR systems, Gentier96E/ Gentier96R/Gentier48E/ Gentier48R Real-time PCR System from Xi'an Tianlong Science and Technology Co., Ltd. |
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