Product Name:
Coxsackie A6/A10 Nucleic Acid Detection Kit (Fluorescence PCR Method)
Spec:
32T/Kit
Intended Use:
The kit is used for in vitro qualitative detection of CA6 nucleic acid and CA10 nucleic acid in human throat swab samples.
Coxsackie is an enterovirus divided into two categories: A and B, among which there are 23 types of type A (A) and 6 types of type B (B). All of them are highly infectious. Coxsackie is a common type of virus that infects human body through respiratory and digestive tract. Infected people will appear fever, sneezing, coughing and other cold symptoms.
The laboratory tests of coxsackie include:
1) Coxsackie isolation and detection;
2) Coxsackie specific marker detection;
3) Serological diagnostic test, etc.
This kit uses real-time fluorescent quantitative PCR method.
Principle of Test:
The highly conserved sequences of CA6 and CA10 are selected to design specific primers and Taqman probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5'end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3'end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of CA6/CA10 nucleic acid in closed reaction system.
A pair of housekeeping gene-specific primers are selected as the internal standard in the kit and combined with specific probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. The internal control adopts TEXAS RED channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negatives.
Storage Condition & Validity:
All reagents should be stored at -20ºC±5 ºC. The validity period of the reagents is 12 months.
Applicable Instrument:
Gentier 96E real time PCR system and Gentier 96R real time PCR system, TL988-IV real time PCR system, ABI7500 real time PCR systems
Sample Requirements :
1. Sample: human throat swab samples.
2. Collection container: a disposable throat swab sampler approved by the state should be selected.
3. Collection: for specific sampling method, please refer to the "Microbial Specimen Collection Manual".
4. Storage: samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for long-time, but repeated freeze-thaw should be avoided.
5. Transportation: use a foam box with ice to seal for transportation.
Tianlong Real Time PCR Test Kit Catalogue:
Tianlong Product Pipeline:
Audited Supplier 
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